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Image Search Results
Journal: Gut microbes
Article Title: Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila .
doi: 10.1080/19490976.2024.2447834
Figure Lengend Snippet: Figure 5. Lactate and A. muciniphila regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of BHI medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Article Snippet: Akkermansia muciniphila (A. muciniphila, ATCC BAA-835) was anaerobically cultured in
Techniques: Western Blot, Expressing, Quantitative RT-PCR, Concentration Assay, Activity Assay
Journal: BMC Microbiology
Article Title: Inhibition of Streptococcus mutans biofilms with bacterial-derived outer membrane vesicles
doi: 10.1186/s12866-021-02296-x
Figure Lengend Snippet: OMVs kill S. mutans biofilm and planktonic bacteria in a time- and dose-dependent manner. S. mutans biofilm cells were harvested from (A) three-day biofilms formed on glass slides cultured in BMGS. Planktonic cells of S. mutans were harvested from culture in BHI broth. S. mutans (B) biofilm cells and (C) planktonic cells were treated with 50 or 100 µg/mL OMVs, 800 µg/mL gentamicin or PBS for up to 24 h with cell viability monitored. Cells were adjusted to start from the same CFU. Bacterial cells under different treatments were plated on agar for CFU determination at different time points. The curves were compared using a Chi-squared test, see . (**** p < 0.0001)
Article Snippet: The suspension was further diluted in
Techniques: Cell Culture